VasoTACS™ In Situ Apoptosis Detection Kit

$385.00

The VasoTACS™ Kit was developed to provide an effective in situ method for identifying apoptosis in vascular samples using an optimized TUNEL based assay.

Available Size(s): 30 Samples Catalog Number: 4826-30-K Category:

Description

This kit allows the user to successfully label apoptotic cells, including endothelial and smooth muscle cells, throughout the vascular system. Similar to the CardioTACS™ Kit, this kit is also based on DNA end-labeling using terminal deoxynucleotidyl transferase (TdT) and a modified nucleotide that is subsequently detected using our TACS Blue Label™ detection system with Red Counterstain C. Specifically, VasoTACS™ has been tested and optimized to help resolve the unique problems encountered when detecting apoptotic vascular cells.

FEATURES:

  • Performance tested on vascular tissues.
  • Includes both Proteinase K and exclusive Cytonin™ non-enzymatic permeabilization reagents.
  • Helps resolve unique problems encountered when detecting apoptotic vascular cells.

APPLICATIONS:

  • In situ detection of apoptosis in fixed frozen, paraffin embedded, or plastic embedded vascular cells and tissues.
  • Assists in the identification of apoptotic morphologies.

 

Protocol(s)

protocol_4826-30-K

Material Safety Data Sheet(s)

msds_4800-30-01 Proteinase K

msds_4800-30-06 Strep-HRP

msds_4800-30-11 TACS-Blue Label

msds_4800-30-12 Blue Strep-HRP Diluent

msds_4800-30-15 TACS-Nuclease

msds_4800-30-16 TACS-Nuclease Buffer

msds_4800-30-19 Red Counterstain C

msds_4810-30-02 10X TdT Labeling Buffer

msds_4810-30-03 10X TdT Stop Buffer

msds_4810-30-04 TdT dNTP Mix

msds_4810-30-05 TdT Enzyme

msds_4810-30-14 50X Manganese Cation

msds_4876-05-01 Cytonin

Catalog # 4826-30-K includes :
Catalog Number Description Qty
4800-30-01 Proteinase K 1
4800-30-06 Streptavidin-HRP 1
4800-30-11 TACS-Blue Label™ 1
4800-30-12 Blue Strep-HRP Diluent 1
4800-30-15 TACS-Nuclease™ 1
4800-30-16 TACS-Nuclease™ Buffer 1
4800-30-19 Red Counterstain C 1
4810-30-02 TACS 2 TdT Labeling Buffer 1
4810-30-03 TACS 2 TdT Stop Buffer 1
4810-30-04 TACS 2 TdT dNTP 1
4810-30-05 TdT Enzyme 1
4810-30-14 50x Manganese Cation 1
4876-05-01 Cytonin™ 1

 

Endothelial lipid phosphate phosphatase-3 deficiency that disrupts the endothelial barrier function is a modifier of cardiovascular development
Ishita Chatterjee, Jugajyoti Baruah, Erin E. Lurie, and Kishore K. Wary
Cardiovasc Res, May 2016; 10.1093/cvr/cvw090.
http://cardiovascres.oxfordjournals.org/cgi/content/abstract/cvw090v2

“Endothelial lipid phosphate phosphatase-3 deficiency that disrupts the endothelial barrier function is a modifier of cardiovascular
development”

Ishita Chatterjee, Jugajyoti Baruah, Erin E. Lurie, and Kishore K. Wary
Cardiovasc Res, Jul 2016; 111: 105 – 118
http://cardiovascres.oxfordjournals.org/cgi/content/abstract/111/1/105