TiterTACS™ Colorimetric Apoptosis Detection Kit

$396.00

The TiterTACS™ Colorimetric Apoptosis Detection Kit is a TUNEL based assay taking advantage of Trevigen’s exclusive in situ labeling technology bringing it to the 96 well microplate format for high throughput quantitative detection of apoptosis.

Available Size(s): 96 Samples Catalog Number: 4822-96-K Category:

Description

During the process of apoptosis, DNA fragmentation occurs following the activation of endonucleases. The labeling of the 3’ ends of DNA fragments provides an easy measure of cells undergoing apoptosis. Modified nucleotides are incorporated at the 3’ ends by the activity of terminal deoxynucleotidyl transferase (TdT). These nucleotides are detected using a horseradish-peroxidase detection system and TACS-Sapphire™. TiterTACS™ can be used with suspension or monolayer cells. The kit is designed to use fixed samples, allowing you to work safely with samples that are infected with biohazardous agents. Fixed samples may be stored conveniently during time-course experiments. The TiterTACS™ Kit also provides TACS-Nuclease™ solution to generate positive controls from your own samples giving you a maximal value for the assay.

Detection using TACS-Sapphire™, a non-toxic colorimetric substrate, allows both kinetic and endpoint readings. The reaction generates a blue product that can be measured at 630 nm, and will turn yellow after the reaction is stopped with acid allowing endpoint reading at 450 nm.

FEATURES:

  • Convenient, 96 well microplate format.
  • Quantitative colorimetric detection.
  • Includes both Proteinase K and exclusive Cytonin™ non-enzymatic permeabilization reagents.
  • Includes TACS-Nuclease solution for preparing sample-dependent positive controls.

APPLICATIONS:

Identification and quantitation of apoptosis in cultured cells in a 96 well assay.

Protocol(s)

protocol_4822-96-K

Material Safety Data Sheet(s)

msds_4800-30-01 Proteinase K

msds_4800-30-06 Strep-HRP

msds_4800-30-12 Blue Strep-HRP Diluent

msds_4800-30-15 TACS-Nuclease

msds_4800-30-16 TACS-Nuclease Buffer

msds_4810-30-04 TdT dNTP Mix

msds_4810-30-14 50X Manganese Cation

msds_4817-60-02 10X TdT Labeling Buffer

msds_4817-60-03 10X TdT Stop Buffer

msds_4821-96-05 TdT Enzyme

msds_4822-96-08 TACS-Sapphire

msds_4876-05-01 Cytonin

Catalog # 4822-96-K includes :
Catalog Number Description Qty
4800-30-01 Proteinase K 2
4800-30-06 Streptavidin-HRP 1
4800-30-12 Blue Strep-HRP Diluent 1
4800-30-15 TACS-Nuclease™ 1
4800-30-16 TACS-Nuclease™ Buffer 1
4810-30-04 TACS 2 TdT dNTP 1
4810-30-14 50x Manganese Cation 2
4817-60-02 10X TdT Labeling Buffer 1
4817-60-03 10X TdT Stop Buffer 1
4821-96-05 TdT Enzyme 1
4822-96-08 TACS-Sapphire™ 1
4876-05-01 Cytonin™ 1

 

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“Ayotunde O. Dokun, Lingdan Chen, Mitsuharu Okutsu, Charles R. Farber, Surovi Hazarika, W. Schuyler
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http://ajpheart.physiology.org/cgi/content/abstract/309/5/H790

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Jintaek Im, Kyutae Kim, Ji Young Yhee, Scott M. O’Grady, and Richard S. Nho PHY2, Nov 2016; 4: e13020.
http://physreports.physiology.org/cgi/content/abstract/4/21/e13020