TACS® 2 TdT DAB (diaminobenzidine) Kit

$385.00

The TACS® 2 TdT DAB Kit utilizes Trevigen’s unique cation optimization system to enhance the in situ detection of apoptosis in a TUNEL based assay within fixed frozen, paraffin embedded, or plastic embedded cells and tissues. Enzymatic incorporation of biotinylated nucleotides in fragmented DNA is performed by a highly purified form of the TdT enzyme in a non-toxic labeling buffer (sodium cacodylate free). Biotin labeling is detected using streptavidin-horseradish peroxidase, and colorimetric substrates diaminobenzidine (DAB).  Samples are counterstained with Methyl Green offering a nice contrast when visualized by light microscopy.

Available Size(s): 30 Samples Catalog Number: 4810-30-K Category:

Description

This complete kit provides all the reagents required for labeling including two permeabilization reagents, labeling and detection reagents, stop buffers, counterstain, and TACS-Nuclease™ reagents for generating positive controls with your own samples. Please refer to our components listing for details.

FEATURES:

  • TdT based in situ labeling.
  • Cation optimization for high signal and low background.
  • Complete kit providing DAB and Methyl Green Counterstain.
  • Includes both Proteinase K and exclusive Cytonin™ non-enzymatic permeabilization reagent.
  • Includes TACS-Nuclease™ control reagents.

APPLICATIONS:

  • In situ detection of apoptosis by TUNEL-based assay in fixed frozen, paraffin embedded, or plastic embedded cells and tissues.
  • Light microscopy.

 

Protocol(s)

protocol_4810-30-K

Material Safety Data Sheet(s)

msds_4800-30-01 Proteinase K

msds_4800-30-06 Strep-HRP

msds_4800-30-07 Diaminobenzidine

msds_4800-30-09 DAB Enhancer Reagent

msds_4800-30-15 TACS-Nuclease

msds_4800-30-16 TACS-Nuclease Buffer

msds_4800-30-18 1.0 Methyl Green

msds_4810-30-02 10X TdT Labeling Buffer

msds_4810-30-03 10X TdT Stop Buffer

msds_4810-30-04 TdT dNTP Mix

msds_4810-30-05 TdT Enzyme

msds_4810-30-09 50X Cobalt Cation

msds_4810-30-10 50X Magnesium Cation

msds_4810-30-14 50X Manganese Cation

msds_4876-05-01 Cytonin

Catalog # 4810-30-K includes :
Catalog Number Description Qty
4800-30-01 Proteinase K 1
4800-30-07 Diaminobenzidine 1
4800-30-09 DAB Enhancer 1
4800-30-15 TACS-Nuclease™ 1
4810-30-04 TACS® 2 TdT dNTP 1
4810-30-05 TdT Enzyme 1
4810-30-09 50x Cobalt Cation 1
4810-30-10 50x Magnesium Cation 1
4810-30-14 50x Manganese Cation 1
4800-30-06 Streptavidin-HRP 1
4800-30-16 TACS-Nuclease™ Buffer 1
4800-30-18 Methyl Green 1% 1
4810-30-02 TACS® 2 TdT Labeling Buffer 1
4810-30-03 TACS® 2 TdT Stop Buffer 1
4876-05-01 Cytonin™ 1

 

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