TACS® 2 TdT Core Kit

The TACS® 2 TdT Core Kit is for researchers familiar with in situ labeling who are already set up for immunohistochemistry. The core kit comes with a streptavidin-HRP conjugate but without an HRP-substrate or a counterstain.

Available Size(s): 30 Samples Catalog Number: 4810-30-CK Category:

Description

TACS® 2 TdT Core kit utilize Trevigen’s unique cation optimization system to enhance labeling within particular tissues. The kit also features a proprietary labeling buffer that contains no toxic components (e.g. sodium cacodylate). A highly purified form of the TdT enzyme is included for the enzymatic incorporation of biotinylated nucleotides. Biotin labeling is detected using streptavidin-horseradish peroxidase, and the researcher’s choice of colorimetric substrates or a fluorescent conjugate of streptavidin may be used for visualization by epifluorescence microscopy.

FEATURES:

  • TdT based in situ labeling.
  • Cation optimization for high signal and low background.
  • Includes exclusive Cytonin™ permeabilization reagent.
  • Includes TACS-Nuclease™ control reagents.
  • Light microscopy or Fluorescence microscopy options.

APPLICATIONS:

  • In situ detection of apoptosis by TUNEL in fixed frozen, paraffin embedded, or plastic embedded cells and tissues.

Protocol(s)

protocol_4810-30-CK and R

Material Safety Data Sheet(s)

msds_4800-30-06 Strep-HRP

msds_4810-30-02 10X TdT Labeling Buffer

msds_4810-30-03 10X TdT Stop Buffer

msds_4810-30-04 TdT dNTP Mix

msds_4810-30-05 TdT Enzyme

msds_4810-30-09 50X Cobalt Cation

msds_4810-30-10 50X Magnesium Cation

msds_4810-30-14 50X Manganese Cation

Catalog # 4810-30-CK includes :
Catalog Number Description Qty
4800-30-06 Streptavidin-HRP 1
4810-30-02 TACS® 2 TdT Labeling Buffer 1
4810-30-03 TACS® 2 TdT Stop Buffer 1
4810-30-04 TACS® 2 TdT dNTP 1
4810-30-05 TdT Enzyme 1
4810-30-09 50x Cobalt Cation 1
4810-30-10 50x Magnesium Cation 1
4810-30-14 50x Manganese Cation 1

 

Autophagy in Pancreatic Acinar Cells in Caerulein-treated Mice: Immunolocalization of Related Proteins and Their Potential as Markers of Pancreatitis
Zhang, L., Zhang, J., Shea, L., Xu, L., Tobin, G., Knapton, A., Sharron, S., and Rouse, R.
Toxicol Pathol, Feb 2014; 42: 435 – 457.
http://tpx.sagepub.com/cgi/content/abstract/42/2/435

Smad7 induces plasticity in tumor-infiltrating Th17 cells and enables TNF-alpha-mediated killing of colorectal cancer cells

Rizzo, A., De Mare, V., Rocchi, C., Stolfi, C., Colantoni, A., Neurath, M.F., Macdonald, T.T., Pallone, F., Monteleone, G., Fantini, M.C.,
Carcinogenesis, Feb 2014; 10.1093/carcin/bgu027.
http://carcin.oxfordjournals.org/cgi/content/abstract/bgu027v2

Differential effects of 2- and 3-series E-prostaglandins on in vitro expansion of Lgr5+ colonic stem cells
Fan, Y.Y., Davidson, L.A.,Callaway, E.S., Goldsby, J.S. and Chapkin, R.S.
Carcinogenesis, Mar 2014; 35: 606-612
http://carcin.oxfordjournals.org/cgi/content/abstract/35/3/606