TACS® 2 TdT Core kit utilize Trevigen’s unique cation optimization system to enhance labeling within particular tissues. The kit also features a proprietary labeling buffer that contains no toxic components (e.g. sodium cacodylate). A highly purified form of the TdT enzyme is included for the enzymatic incorporation of biotinylated nucleotides. Biotin labeling is detected using streptavidin-horseradish peroxidase, and the researcher’s choice of colorimetric substrates or a fluorescent conjugate of streptavidin may be used for visualization by epifluorescence microscopy.
- TdT based in situ labeling.
- Cation optimization for high signal and low background.
- Includes exclusive Cytonin™ permeabilization reagent.
- Includes TACS-Nuclease™ control reagents.
- Light microscopy or Fluorescence microscopy options.
- In situ detection of apoptosis by TUNEL in fixed frozen, paraffin embedded, or plastic embedded cells and tissues.
Autophagy in Pancreatic Acinar Cells in Caerulein-treated Mice: Immunolocalization of Related Proteins and Their Potential as Markers of Pancreatitis
Zhang, L., Zhang, J., Shea, L., Xu, L., Tobin, G., Knapton, A., Sharron, S., and Rouse, R.
Toxicol Pathol, Feb 2014; 42: 435 – 457.
Smad7 induces plasticity in tumor-infiltrating Th17 cells and enables TNF-alpha-mediated killing of colorectal cancer cells
Rizzo, A., De Mare, V., Rocchi, C., Stolfi, C., Colantoni, A., Neurath, M.F., Macdonald, T.T., Pallone, F., Monteleone, G., Fantini, M.C.,
Carcinogenesis, Feb 2014; 10.1093/carcin/bgu027.
Differential effects of 2- and 3-series E-prostaglandins on in vitro expansion of Lgr5+ colonic stem cells
Fan, Y.Y., Davidson, L.A.,Callaway, E.S., Goldsby, J.S. and Chapkin, R.S.
Carcinogenesis, Mar 2014; 35: 606-612