TACS® 2 TdT Blue Label Kit

$385.00

The TACS® 2 TdT Blue Label Kit utilizes Trevigen’s unique cation optimization system to enhance in situ detection of apoptosis (by TUNEL) in fixed frozen, paraffin embedded, or plastic embedded cells and tissues. Enzymatic incorporation of biotinylated nucleotides in fragmented DNA is performed by a highly purified form of the TdT enzyme in a non-toxic labeling buffer (sodium cacodylate free). Biotin labeling is detected using streptavidin-horseradish peroxidase, and colorimetric substrate TACS Blue Label™.  Samples are counterstained with Nuclear Fast Red offering a nice contrast when visualized by light microscopy.

Available Size(s): 30 Samples Catalog Number: 4811-30-K Category:

Description

This complete kit provides all the reagents required for labeling including two permeabilization reagents, labeling and detection reagents, stop buffers, counterstain, and TACS-Nuclease™ reagents for generating positive controls with your own samples. Please refer to our components listing for details.

FEATURES:

  • TdT based in situ labeling.
  • Cation optimization for high signal and low background.
  • Includes both Proteinase K and exclusive Cytonin™ non-enzymatic permeabilization reagent.
  • Includes TACS-Nuclease™ control reagents.
  • Dark blue labeling with red counterstain.

APPLICATIONS:

  • In situ detection of apoptosis in fixed frozen, paraffin embedded, or plastic embedded cells and tissues.
  • Light microscopy.

Protocol(s)

protocol_4811-30-K

Material Safety Data Sheet(s)

msds_4800-30-01 Proteinase K

msds_4800-30-06 Strep-HRP

msds_4800-30-11 TACS-Blue Label

msds_4800-30-12 Blue Strep-HRP Diluent

msds_4800-30-15 TACS-Nuclease

msds_4800-30-16 TACS-Nuclease Buffer

msds_4800-30-17 Nuclear Fast Red

msds_4810-30-02 10X TdT Labeling Buffer

msds_4810-30-03 10X TdT Stop Buffer

msds_4810-30-04 TdT dNTP Mix

msds_4810-30-05 TdT Enzyme

msds_4810-30-09 50X Cobalt Cation

msds_4810-30-10 50X Magnesium Cation

msds_4810-30-14 50X Manganese Cation

msds_4876-05-01 Cytonin

Catalog # 4811-30-K includes :
Catalog Number Description Qty
4800-30-01 Proteinase K 1
4800-30-06 Streptavidin-HRP 1
4800-30-11 TACS-Blue Label™ 1
4800-30-12 Blue Strep-HRP Diluent 1
4800-30-15 TACS-Nuclease™ 1
4800-30-16 TACS-Nuclease™ Buffer 1
4800-30-17 Nuclear Fast Red 1
4810-30-04 TACS® 2 TdT dNTP 1
4810-30-05 TdT Enzyme 1
4810-30-09 50x Cobalt Cation 1
4810-30-10 50x Magnesium Cation 1
4810-30-14 50x Manganese Cation 1
4810-30-02 TACS® 2 TdT Labeling Buffer 1
4810-30-03 TACS® 2 TdT Stop Buffer 1
4876-05-01 Cytonin™ 1

 

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