NeuroTACS™ II In Situ Apoptosis Detection Kit

NeuroTACS™ II is a complete  kit optimized to provide rapid and convenient identification of apoptosis in brain tissue or neuronal cells using a TUNEL based assay.

Available Size(s): 30 Samples Catalog Number: 4823-30-K Category:

Description

The kit has been developed to overcome the common difficulties unique to neuronal samples including the fragile nature of brain tissue sections, high background problems, poor counterstaining with common dyes, and the need to perform dual labeling experiments to detect cell specific antigens in conjunction with apoptotic cells. A key feature is NeuroPore™, a proprietary permeabilization reagent that gently permeabilizes samples while retaining cell morphology. NeuroPore™ also contains blocking reagents to allow its use as an antibody diluent in immunohistochemistry and to reduce background staining.

DNA fragments generated by apoptosis are end-labeled with modified nucleotides using a highly purified terminal deoxynucleotidyl transferase enzyme (TdT). The incorporated nucleotides are detected using a horseradish peroxidase system that catalyzes the conversion of diaminobenzidine (DAB) into a visible dark brown precipitate. NeuroTACS™ II contains the Blue Counterstain to allow visualization of all cells within the sample with good contrast to the brown DAB precipitate. A DAB enhancer is also provided with the kit for the option to intensify or darken DAB staining. In addition, the Blue Counterstain is compatible with the red-colored substrates used for phosphatase detection in double labeling experiments. The protocol includes details for labeling in situ for apoptosis and antigen detection on the same sample. To ensure that your own samples have been processed and permeabilized correctly, we provide TACS-Nuclease™, a unique reagent used to generate a positive control with your own samples. This provides a high degree of confidence for data interpretation and can help pinpoint problem steps in the labeling procedure.

FEATURES:

  • Unique buffer system produces more consistent labeling.
  • Performance tested on brain sections.
  • Includes exclusive NeuroPore™ permeabilization reagent.
  • Includes TACS-Nuclease™ solution for preparing sample-dependent positive controls.
  • Helps resolve unique problems encountered when detecting apoptotic neuronal cells.

 

APPLICATIONS:

  • In situ detection of apoptosis in fixed frozen, paraffin embedded, or plastic embedded cells and tissues.
  • Assists in the identification of apoptotic morphologies.

Protocol(s)

protocol_4823-30-K

Material Safety Data Sheet(s)

msds_4800-30-01 Proteinase K

msds_4800-30-06 Strep-HRP

msds_4800-30-07 Diaminobenzidine

msds_4800-30-09 DAB Enhancer Reagent

msds_4800-30-15 TACS-Nuclease

msds_4800-30-16 TACS-Nuclease Buffer

msds_4810-30-02 10X TdT Labeling Buffer

msds_4810-30-03 10X TdT Stop Buffer

msds_4810-30-04 TdT dNTP Mix

msds_4810-30-05 TdT Enzyme

msds_4810-30-14 50X Manganese Cation

msds_4820-30-13 Blue Counterstain

msds_4876-05-01 Cytonin

Catalog # 4823-30-K includes :
Catalog Number Description Qty
4800-30-01 Proteinase K 1
4800-30-06 Streptavidin-HRP 1
4800-30-07 Diaminobenzidine 1
4800-30-09 DAB Enhancer 1
4800-30-15 TACS-Nuclease™ 1
4800-30-16 TACS-Nuclease™ Buffer 1
4810-30-02 TACS® 2 TdT Labeling Buffer 1
4810-30-03 TACS® 2 TdT Stop Buffer 1
4810-30-04 TACS® 2 TdT dNTP 1
4810-30-05 TdT Enzyme 1
4810-30-14 50x Manganese Cation 1
4820-30-01 NeuroPore™ 1
4820-30-13 Blue Counterstain 1

 

Differences in the Phagocytic Response of Microglia and Peripheral Macrophages after Spinal Cord Injury and Its Effects on Cell Death,
Andrew D. Greenhalgh and Samuel David
J. Neurosci., Apr 2014; 34: 6316 – 6322.
http://www.jneurosci.org/cgi/content/abstract/34/18/6316

Hypoxia Inducible Factor-1{alpha} (HIF-1{alpha}) Is Required for Neural Stem Cell Maintenance and Vascular Stability in the Adult Mouse SVZ
Lu Li, Kate M. Candelario, Kelsey Thomas, Ruth Wang, Kandis Wright, Amber Messier, and Lee Anna Cunningham

Synaptic Synthesis, Dephosphorylation, and Degradation: A NOVEL PARADIGM FOR AN ACTIVITY-DEPENDENT NEURONAL CONTROL OF CDKL5

Paolo La Montanara, Laura Rusconi, Albina Locarno, Lia Forti, Isabella Barbiero, Marco Tramarin, Chetan Chandola, Charlotte Kilstrup-Nielsen, and Nicoletta Landsberger
J. Biol. Chem., Feb 2015; 290: 4512 – 4527.
http://www.jbc.org/cgi/content/abstract/290/7/4512