FlowTACS™ In Situ Apoptosis Detection Kit

$385.00

FlowTACS™  takes advantage of Trevigen’s exclusive in situ labeling technology to identify and quantitate cultured apoptotic cells by TUNEL-based and Flow Cytometry technologies.

Available Size(s): 60 Samples Catalog Number: 4817-60-K Category:

Description

FlowTACS™ also provides flexibility in selection of fluorophores that are compatible with your research design. The kit allows multi-color labeling in conjunction with experiment-specific antibodies.

DNA fragmentation is an irreversible, committed step in apoptosis, and the labeling of 3’ ends provides an easy measure of cells undergoing apoptosis. Cells may also be analyzed for DNA content using the included propidium iodide/RNase A solution. The FlowTACS™ Kit also provides TACS-Nuclease™ to generate positive controls for calibrating your flow cytometer. The FlowTACS™ Kit uses fixed cells, allowing you to store samples during time-course experiment and to safely work with biohazardous agents.

This complete kit provides all the reagents required for labeling including two permeabilization reagents, labeling and stop buffers, labeling and detection reagents, and TACS-Nuclease™ for generating positive controls with your own samples. Please refer to our components listing for details.

FEATURES:

  • Designed for use with fixed cells.
  • Includes exclusive non-enzymatic Cytonin™ permeabilization reagent.
  • Includes TACS-Nuclease™ solution for preparing sample-dependent positive controls.

APPLICATIONS:

  • Identification and quantitation of fixed apoptotic cells in suspension by Flow Cytometry.
  • Allows bi-color and tri-color analysis.

Protocol(s)

protocol_4817-60-K

Material Safety Data Sheet(s)

msds_4800-30-14 Strep-Fluorescein

msds_4800-30-15 TACS-Nuclease

msds_4800-30-16 TACS-Nuclease Buffer

msds_4810-30-04 TdT dNTP Mix

msds_4810-30-05 TdT Enzyme

msds_4810-30-14 50X Manganese Cation

msds_4817-60-02 10X TdT Labeling Buffer

msds_4817-60-03 10X TdT Stop Buffer

msds_4817-60-04 Propidium Iodide RNase

msds_4876-05-01 Cytonin

Catalog # 4817-60-K includes :
Catalog Number Description Qty
4800-30-14 Streptavidin-FITC 1
4800-30-15 TACS-Nuclease™ 1
4800-30-16 TACS-Nuclease™ Buffer 1
4810-30-04 TACS® 2 TdT dNTP 1
4810-30-05 TdT Enzyme 1
4810-30-14 50x Manganese Cation 1
4817-60-02 10X TdT Labeling Buffer 1
4817-60-03 10X TdT Stop Buffer 1
4817-60-04 PI/RNase 1
4876-05-01 Cytonin™ 1