CardioTACS™ In Situ Apoptosis Detection Kit

$385.00

The CardioTACS™ Kit was developed to provide the cardiac researcher with an effective method for identifying apoptotic cells in cardiac samples using an optimized TUNEL based assay.

Available Size(s): 30 Samples Catalog Number: 4827-30-K Category:

Description

The high cellularity of cardiac tissue presents problems in permeabilization, so the CardioTACS™ Kit comes with two permeabilization reagents to provide options. The kit is based on DNA end-labeling using terminal deoxynucleotidyl transferase (TdT) and a modified nucleotide that is subsequently detected using our TACS Blue Label™ detection system. Trevigen has developed an exclusive apoptosis grade TdT enzyme that when coupled with the TACS Blue Label™ solution, provides labeling 20 to 50 times more sensitive than standard diaminobenzidine (DAB) labeling methods. To ensure ease of data interpretation when the numbers of apoptotic cells are low, CardioTACS™ includes Nuclear Fast Red Counterstain which provides superb contrast to the TACS Blue Label™ in apoptotic cells.

In addition, the kit includes TACS-Nuclease™ to generate a positive control in your own sample. The positive control provides an internal control for permeabilization and labeling so additional optimization is nominal and the data can be interpreted with confidence. This complete kit provides all the reagents required for labeling including two permeabilization reagents, labeling and detection reagents, stop buffer, counterstain, TACS-Nuclease™ reagent, and a protocol detailing incubation times and reagent concentrations.

 

FEATURES:

  • Performance tested on heart-derived samples.
  • Includes both Proteinase K and exclusive Cytonin™ non-enzymatic permeabilization reagents.
  • Includes TACS-Nuclease™ solution for preparing sample-dependent positive controls.
  • Helps resolve unique problems encountered when detecting apoptotic cardiac cells.

APPLICATIONS:

  • In situ detection of apoptosis in fixed frozen, paraffin embedded, or plastic embedded cardiac cells and tissues.
  • Assists in the identification of apoptotic morphologies.

Protocol(s)

protocol_4827-30-K

Material Safety Data Sheet(s)

msds_4800-30-01 Proteinase K

msds_4800-30-06 Strep-HRP

msds_4800-30-11 TACS-Blue Label

msds_4800-30-12 Blue Strep-HRP Diluent

msds_4800-30-15 TACS-Nuclease

msds_4800-30-16 TACS-Nuclease Buffer

msds_4800-30-17 Nuclear Fast Red

msds_4810-30-02 10X TdT Labeling Buffer

msds_4810-30-03 10X TdT Stop Buffer

msds_4810-30-04 TdT dNTP Mix

msds_4810-30-05 TdT Enzyme

msds_4810-30-14 50X Manganese Cation

msds_4876-05-01 Cytonin

Catalog # 4827-30-K includes :
Catalog Number Description Qty
4800-30-01 Proteinase K 1
4800-30-06 Streptavidin-HRP 1
4800-30-11 TACS-Blue Label™ 1
4800-30-12 Blue Strep-HRP Diluent 1
4800-30-15 TACS-Nuclease™ 1
4800-30-16 TACS-Nuclease™ Buffer 1
4800-30-17 Nuclear Fast Red 1
4810-30-02 TACS® 2 TdT Labeling Buffer 1
4810-30-03 TACS® 2 TdT Stop Buffer 1
4810-30-04 TACS® 2 TdT dNTP 1
4810-30-05 TdT Enzyme 1
4810-30-14 50x Manganese Cation 1
4876-05-01 Cytonin™ 1

 

The Influence of Age on Serum Concentrations of Cardiac Troponin I: Results in Rats, Monkeys, and Commercial Sera
Eugene H. Herman, Alan Knapton, Yongmin Liu, Steven E. Lipshultz, Joel Estis, John Todd, Ruth A. Woodward, Thomas Cochran, Jun Zhang, and Miriam C. Poirier
Toxicol Pathol, Jul 2014; 42: 888 – 896.
http://tpx.sagepub.com/cgi/content/abstract/42/5/888

“GSK-3β heterozygous knockout is cardioprotective in a knockin mouse
model of familial dilated cardiomyopathy”

“Rasha M. S. M. Mohamed, Sachio Morimoto, Islam A. A. E.-H. Ibrahim, Dong-Yun Zhan, Cheng-Kun Du,Masaki Arioka, Tatsuya Yoshihara, Fumi Takahashi-Yanaga, and Toshiyuki Sasaguri”
Am J Physiol Heart Circ Physiol, Jun 2016; 310: H1808 – H1815
http://ajpheart.physiology.org/cgi/content/abstract/310/11/H1808

“Phosphodiesterase 5 Inhibition Limits Doxorubicin-induced Heart Failure by Attenuating Protein Kinase G Iα Oxidation”
“Oleksandra Prysyazhna, Joseph Robert Burgoyne, Jenna Scotcher, Steven Grover, David Kass, and Philip Eaton”
J. Biol. Chem., Aug 2016; 291: 17427 – 17436.
http://www.jbc.org/cgi/content/abstract/291/33/17427