MTT Reagent is supplied ready to use and is stable when stored at 4°C.
Changes in cell proliferative activity caused by trophic factors, growth inhibitors, or inducers and inhibitors of apoptosis, may be quantified using the MTT Cell Proliferation Assay Kit. MTT is reduced to an insoluble formazan dye by mitochondrial enzymes associated with metabolic activity. The reduction of MTT is primarily due to glycolytic activity within the cell and is dependent upon the presence of NADH and NADPH. The resultant intracellular purple formazan can be solubilized and quantitated by spectroscopic means.