MTT Cell Proliferation Assay Kit

The TACS® MTT Cell Proliferation Assay (MTT-CPA) is a sensitive kit for the measurement of cell proliferation based upon the reduction of the tetrazolium salt, 3,[4,5-dimethylthiazol-2- yl]-2,5-diphenyl-tetrazolium bromide (MTT).

Available Size(s): 2500 Wells, 5000 Wells Catalog Number: 4890-25-K Category:

Description

Changes in cell proliferative activity caused by trophic factors, growth inhibitors, or inducers and inhibitors of apoptosis, may be quantified using the MTT-CPA. MTT is reduced to an insoluble formazan dye by mitochondrial enzymes associated with metabolic activity. The reduction of MTT is primarily due to glycolytic activity within the cell and is dependent upon the presence of NADH and NADPH. The resultant intracellular purple formazan can be solubilized and quantitated by spectroscopic means.

Features:

  • Allows measurement of proliferation rate, and conversely, the reduction in cell viability.
  • Minimizes the number of steps necessary to complete the assay and interpret the data.
  • Yields low background absorbance values in the absence of cells.
  • Accurate quantification of changes in cell proliferation due to the linear relationship between cell number and signal produced for each cell type.
  • Stable when stored at 4°C.

Protocol(s)

protocol_4890-025-K & 4890-050-K

Material Safety Data Sheet(s)

msds_4890-25-01 MTT Reagent

msds_4890-25-02 Detergent Reagent

Catalog # 4890-50-K includes :
Catalog Number Description Qty
4890-25-01 MTT Reagent 2
4890-25-02 Detergent Reagent 2
Catalog # 4890-25-K includes :
Catalog Number Description Qty
4890-25-01 MTT Reagent 1
4890-25-02 Detergent Reagent 1

 

Identification of Chemical Compounds That Inhibit the Function of Glutamyl-tRNA Synthetase from Pseudomonas aeruginosa
Yanmei Hu, Edgar Guerrero, Megan Keniry, Joel Manrrique, and James M. Bullard
J Biomol Screen, Jun 2015; 10.1177/1087057115591120.
http://jbx.sagepub.com/cgi/content/abstract/1087057115591120v1

Characterization of CM572, a Selective Irreversible Partial Agonist of the Sigma-2 Receptor with Antitumor Activity
Hilary Nicholson, Anthony Comeau, Christophe Mesangeau, Christopher R. McCurdy, and Wayne D. Bowen
J. Pharmacol. Exp. Ther., Jul 2015; 354: 203 – 212.
http://jpet.aspetjournals.org/cgi/content/abstract/354/2/203

“Imidazolium Salts as Small-Molecule Urinary Bladder Exfoliants in a
Murine Model”

“Patrick O. Wagers, Kristin M. Tiemann, Kerri L. Shelton, William G. Kofron, Matthew J. Panzner, Karen L.
Wooley, Wiley J. Youngs, and David A. Hunstad”
Antimicrob. Agents Chemother., Sep 2015; 59: 5494 – 5502
http://aac.asm.org/cgi/content/abstract/59/9/5494

“Identification of Chemical Compounds That Inhibit the Function of Glutamyl-tRNA Synthetase from Pseudomonas aeruginosa”
Yanmei Hu, Edgar Guerrero, Megan Keniry, Joel Manrrique, and James M. Bullard
J Biomol Screen, Oct 2015; 20: 1160 – 1170.
http://jbx.sagepub.com/cgi/content/abstract/20/9/1160