Calcein AM

Calcein AM is a cell-permeant dye that can be used to determine cell viability and/or cytotoxicity in most eukaryotic cells. Calcein AM is also available as a component of the Calcein AM Cell Viability Kit.

 

Available Size(s): 50 ug Catalog Number: 4892-010-01 Category:

Description

Calcein AM (structure A) is a non-fluorescent, hydrophilic compound that easily permeates intact, live cells. The hydrolysis of Calcein AM by intracellular esterases produces calcein (structure B), a hydrophilic, strongly fluorescent compound that is well-retained in the cell cytoplasm. Cells grown in black-walled plates can be stained and quantified in less than two hours.

Specifications:

    • Molecular weight: 995
    • Presentation: 50 μg dessicated pellet
    • Soluble in DMSO

 

Protocol(s)

Part of below kit(s)

protocol_4892-010-K

Material Safety Data Sheet(s)

msds_4892-010-01 Calcein AM

Metastasis Suppressors Regulate the Tumor Microenvironment by Blocking Recruitment of Prometastatic Tumor-Associated Macrophages
Casey Frankenberger, Daniel Rabe, Russell Bainer, Devipriya Sankarasharma, Kiran Chada, Thomas Krausz, Yoav Gilad, Lev Becker, and Marsha Rich Rosner
Cancer Res., Oct 2015; 75: 4063 – 4073.
http://cancerres.aacrjournals.org/cgi/content/abstract/75/19/4063