Calcein AM Cell Viability Assay Kit

The Calcein AM Kit provides a simple, rapid and accurate method to measure cell viability and/or cytotoxicity.

Available Size(s): 1000 Wells Catalog Number: 4892-010-K Category:

Description

Calcein AM is a non-fluorescent, hydrophobic compound that easily permeates intact, live cells. The hydrolysis of Calcein AM by intracellular esterases produces calcein, a hydrophilic, strongly fluorescent compound that is well-retained in the cell cytoplasm. Cells grown, preferably in black-walled plates, can be stained and quantified in less than two hours.

Features:

  • Suitable for proliferating and non-proliferating cells.
  • Ideal for both suspension and adherent cells.
  • Non-radioactive assay.
  • Rapid (no solubilization step as in a conventional MTT assay).
  • Ideal for high-throughput experiments.
  • Better retention and brightness compared to other fluorescent compounds (e.g. fluorescein).
  • Useful in a variety of studies, including: cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis, and cytotoxicity.
  • Adaptable to a wide variety of techniques, including: microplate assays, immunocytochemistry, flow cytometry, and in vivo cell tracking.

Protocol(s)

protocol_4892-010-K

Material Safety Data Sheet(s)

msds_4892-010-01 Calcein AM

msds_4892-010-02 10X Calcein AM DW Buffer

 

Catalog # 4892-010-K includes :
Catalog Number Description Qty
4892-010-01 Calcein AM 2
4892-010-02 10X Calcein AM DW Buffer 1

Prolyl-4-hydroxylase domain 3 (PHD3) is a critical terminator for cell survival of macrophages under stress conditions
Lija Swain, Marieke Wottawa, Annette Hillemann, Angelika Beneke, Haruki Odagiri, Kazutoyo Terada, Motoyoshi Endo, Yuichi Oike, Katja Farhat, and Dörthe M. Katschinski
J. Leukoc. Biol., Sep 2014; 96: 365 – 375.
http://www.jleukbio.org/cgi/content/abstract/96/3/365