Calcein AM is a non-fluorescent, hydrophobic compound that easily permeates intact, live cells. The hydrolysis of Calcein AM by intracellular esterases produces calcein, a hydrophilic, strongly fluorescent compound that is well-retained in the cell cytoplasm. Cells grown, preferably in black-walled plates, can be stained and quantified in less than two hours.
- Suitable for proliferating and non-proliferating cells.
- Ideal for both suspension and adherent cells.
- Non-radioactive assay.
- Rapid (no solubilization step as in a conventional MTT assay).
- Ideal for high-throughput experiments.
- Better retention and brightness compared to other fluorescent compounds (e.g. fluorescein).
- Useful in a variety of studies, including: cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis, and cytotoxicity.
- Adaptable to a wide variety of techniques, including: microplate assays, immunocytochemistry, flow cytometry, and in vivo cell tracking.