The need for more complete and physiologically predictive cancer growth and invasion models has driven the development of the 3D Spheroid Assays. There is growing evidence that tumor cell aggregates or spheroids are more representative of tumors in vivo, and they exhibit several physiological traits including similar morphology, the formation of cell-cell bonds, decreased proliferation rates, increased cell survival, tumor dormancy, and a hypoxic core. Applying this model to 3D culture proliferation and invasion assays provides a more physiological approach for assessing tumor growth and invasion.
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