Extracellular Matrix Proteins
To support the investigation of cell behavior in vitro and in vivo, Trevigen offers its Cultrex® line of extracellular matrix proteins. Products include a full range of matrices and purified proteins including Basement Membrane Matrix, Laminin I, Collagen I, Collagen IV, Vitronectin, and Fibronectin. Select Cultrex® products undergo additional qualification for use in 3D cultures or stem cell cultures. Trevigen has initiated state of the art quality, sterility, infectious agent testing and employs the most stringent and rigorous endotoxin specifications in our industry.
3D Culture Qualified (3)3D cell cultures are in vitro cell culture models where cells are suspended in an environment to facilitate growth in all three dimensions. Within tissues, the extracellular matrix (ECM) provides structural and functional organization of resident cells. 3D culture models recapitulate cell-ECM and cell-cell interactions to promote in vivo-like self assembly of cells in vitro to mimic their emanating tissue. Trevigen 3D culture qualified products undergo rigorous 3D testing so that you can be confident that the product will work for your 3D culture application.
Cultrex® BME (9)Cultrex® Basement Membrane Extract (BME), Trevigen's Basement Membrane Matrix, is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor. The matrix gels at 37 °C to form a reconstituted basement membrane. The major components of BME include laminin, collagen IV, entactin, and heparin sulfate proteoglycan. BME can be used in multiple applications, under a variety of cell culture conditions, for maintaining growth or promoting differentiation of primary endothelial, epithelial, smooth muscle and stem cells. BME can also be utilized in cell attachment, neurite outgrowth, angiogenesis, in vitro cell invasion and in vivo tumorigenicity assays. Trevigen has different BME formulations to provide superior alternatives to Matrigel® or Geltrex®.
Matrix Buffer Concentration Applications Cultrex® Original BME DMEM 12-18 mg/ml General (2D Culture, 3-D Culture, Attachment, Invasion, etc.) Cultrex® Stem Cell Qualified BME DMEM 12-18 mg/ml Attachment and Maintenance of Stem Cells Cultrex® 3-D Culture Matrix™ BME DMEM 14-16 mg/ml 3-D Culture Cultrex® Organoid Qualified BME, Type 2 DMEM 12-18 mg/ml Robust Organoid Culture Cultrex® BME, Type 3 RPMI1640 12-18 mg/ml Xenograft/Tumorgraft Cultrex® Organoid Qualified BME, Type R1 DMEM 12-18 mg/ml Difficult to Grow Organoid Culture
Matrigel is a registered trademark of Corning Incorporated. Geltrex is a registered trademark of Invitrogen Corporation.Explore All
Purified Proteins (9)Cultrex® Purified extracellular matrix (ECM) proteins provide a defined substrate for cell adhesion in culture. To accommodate a variety of different cell models Trevigen has developed a full range of ECM component proteins including Laminin I, Collagen I, Collagen IV, Fibronectin, and Vitronectin. These include purified proteins that have been qualified for 3D or stem cell cultures.
Polyamino acids (3)Poly-amino acids are synthetic polymers made up of many repeating units of an amino acid. Cultrex® Poly-L-Lysine, Poly-D-Lysine, and Poly-L-Ornithine may be used as coating agents that promote protein or cell adhesion for tissue culture.
Stem Cell Qualified (4)Trevigen’s Cultrex® stem cell qualified proteins were created to help researchers to choose optimal coating conditions for feeder-free culture of stem cells of interest. These proteins for coating include Mouse Reduced Growth Factor Basement Membrane Matrix, Mouse Laminin I, Human Fibronectin, and Human Vitronectin. Each of these proteins was qualified in feeder-free cell culture to support human stem cells in the undifferentiated state for multiple passages.