Angiogenesis and Vascular Integrity Assays
Angiogenesis is the process by which new capillaries arise from pre-existing vasculature. Pathogenic angiogenesis is a common feature of rheumatoid arthritis, age-related macular degeneration, atherosclerosis and cancer. Cost-effective assays are important tools in the development of new therapeutic strategies to modulate neovascularization. Trevigen has developed several standardized assays for quantifying angiogenesis including Endothelial Invasion Assays, Tube Formation Assays, and Directed In Vivo Angiogenesis Assays (DIVAA).
In Vitro (4)The Cultrex® In Vitro Angiogenesis Assays have been developed for evaluating angiogenesis in vitro. These products utilize PathClear® Basement Membrane Extract (BME), Trevigen’s Basement Membrane Matrix, to provide standardized assays for evaluating endothelial tube formation and endothelial cell invasion. These in vitro angiogenesis assays have been used successfully to identify pro-angiogenic factors and inhibitors.
In Vivo (11)The Directed In Vivo Angiogenesis Assay (DIVAA™) has been developed for evaluating angiogenesis in vivo. This line of products utilize PathClear® Basement Membrane Extract (BME), Trevigen's Basement Membrane Matrix, and DIVAA™ angioreactors, which are small, implant grade silicone cylinders that are sealed on one end, to quantitatively and reproducibly measure in vivo angiogenesis. By encapsulating the angiogenic response, DIVAA™ preserves vascular integrity and prevents absorption of experimental sample, avoiding the pitfalls of the Matrigel Plug Assay.