The adaptation of 3D culture models for studying biochemical processes has been impeded by the challenge of separating intact cells from extracellular matrix (ECM) proteins comprising the hydrogel. Commonly, proteases are employed to degrade these extracellular proteins; however, proteases also degrade proteins on the cell surface and protease activity may carry over into lysate preparations. Trevigen has developed non-enzymatic methodologies for depolymerizing ECM proteins, providing optimized and standardized solutions for separating intact structures from 3D cultures.
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