Cellular responses to oxidative stress are of interest to investigators of a wide variety of human diseases such as cancer, diabetes, atherosclerosis, stroke, Alzheimer’s, many auto-immune diseases, and aging. Oxidative stress occurs when the levels of reactive oxygen species (ROS) increase dramatically in the cell leading to oxidative damage. The estimated frequency of oxidatively-induced DNA lesions is approximately 104 lesions per cell per day. The most abundant DNA lesion generated following exposure to hydroxyl radicals is 8-oxo-dG. Common pollutants such as cigarette smoke also trigger a metabolic response resulting in the formation of bulky DNA adducts (BPDE). Biomarkers frequently used to measure cellular defensive responses against oxidative stress include superoxide dismutase (SOD), glutathione reductase, glutathione peroxidase, and glutathione (GSH).
Trevigen offers products to enable investigators to monitor changes in 8-oxo-dG and BPDE levels: high-throughput ELISA kits for 8-oxo-dG and BPDE including the monoclonal antibodies for detection. Trevigen research kits employ well-established methods to assay the known biomarkers GSH and SOD.
HT 8-oxo-dG ELISA Kit and Monoclonal Antibody (2)
8-oxo-2'-deoxyguanosine (8-oxo-dG), a frequently used biomarker of oxidative DNA damage, is removed from DNA by the base excision repair pathway, and subsequently transported into saliva, urine and plasma. To measure the total pool of 8-oxo-dG, Trevigen developed a validated competitive HT 8-oxo-dG ELISA Kit and monoclonal antibody that quantifies 8-oxo-dG in samples independent of species.
Superoxide Dismutase Assay Kits (2)
Superoxide Dismutases (SOD) catalyze the dismutation of the superoxide radical (O2 -) into hydrogen peroxide (H2O2) and elemental oxygen (O2). This provides an important defense against the toxicity of superoxide radicals. SOD1 and SOD2-deficient mice spontaneously develop liver cancer and are prone to develop tumors, whereas over expression of SOD protects tumor cells from apoptosis. Trevigen offers two different Superoxide Dismutase assay kits to fit your needs.
The HT Superoxide Dismutase 96 well Plate Assay Kit utilizes WST-1, which is converted to WST-1 formazan by xanthine oxidase (XOD). The WST-1 formazan product is read at 450 nm in a plate reader. The Superoxide Dismutase Cuvette Assay Kit employs NBT, which is converted to NBT-diformazan by XOD. The NBT-diformazan product is read at 550 nm in a spectrophotometer. Both assays are free of interference by other catalytic activities, and unlike some other assay kits for SOD, these systems are not greatly disturbed by trace metals. In addition, both kits are ideal for assaying SOD in mammalian cell or tissue lysates. Please click the links below for more information on each assay.
Glutathione Assay Kits (4)
Glutathione (GSH) is an important intracellular tripeptide and a known antioxidant. The thiol group within the cysteine residue acts as a reducing agent to assist reduction enzymes such as glutathione peroxidase to convert the highly reactive hydrogen peroxide to water. In the process, the glutathione is oxidized to glutathione disulfide (GSSG). The reverse reaction takes place using the glutathione reductase enzyme and NADPH as the reducing agent to reduce GSSG to GSH. Trevigen offers several different glutathione assay kits depending on which reaction you wish to study.
The Glutathione Reductase Assay Kit (Cuvette) measures levels of glutathione reductase in cell lysates, erythrocyte lysates, and tissue homogenates. This is the cuvette version of the 7513-500-K.
The HT Glutathione Assay Kit (Total Glutathione) quantifies the amount of glutathione in mammalian cells, tissue, blood, plasma and other bodily fluids using a 96 well plate.
The HT Glutathione Peroxidase Assay Kit measures the amount of glutathione peroxidase in plasma, erythrocyte lysates, tissue homogenates, and cell lysates using a 96 well plate.
The HT Glutathione Reductase Assay Kit (96 Well Plate) measures levels of glutathione reductase in cell lysates, erythrocyte lysates, and tissue homogenates. This is the 96 well plate version of the 7510-100-K.Please select a kit to find out more information.
HT BPDE ELISA Kit and Monoclonal Antibody (2)
Benzo(a)pyrene (BP) is a widespread environmental pollutant found in cigarette smoke, charred food, exhaust from internal combustion engines and coal-burning factories. A highly reactive diol epoxide derivative, BPDE, is the metabolite involved in covalent binding to DNA and proteins. The formation of BPDE adducts is associated with increased risk of lung and breast cancer. Trevigen offers a HT BPDE ELISA Kit and monoclonal antibody that quantifies BPDE adducts in plasma, serum and cell culture supernatant samples.