Superoxide Dismutase Assay Kits
Superoxide Dismutases (SOD) catalyze the dismutation of the superoxide radical (O2 -) into hydrogen peroxide (H2O2) and elemental oxygen (O2). This provides an important defense against the toxicity of superoxide radicals. SOD1 and SOD2-deficient mice spontaneously develop liver cancer and are prone to develop tumors, whereas over expression of SOD protects tumor cells from apoptosis. Trevigen offers two different Superoxide Dismutase assay kits to fit your needs.
The HT Superoxide Dismutase 96 well Plate Assay Kit utilizes WST-1, which is converted to WST-1 formazan by xanthine oxidase (XOD). The WST-1 formazan product is read at 450 nm in a plate reader. The Superoxide Dismutase Cuvette Assay Kit employs NBT, which is converted to NBT-diformazan by XOD. The NBT-diformazan product is read at 550 nm in a spectrophotometer. Both assays are free of interference by other catalytic activities, and unlike some other assay kits for SOD, these systems are not greatly disturbed by trace metals. In addition, both kits are ideal for assaying SOD in mammalian cell or tissue lysates. Please click the links below for more information on each assay.