DNA Repair Enzymes and Reaction Buffers
DNA base excision repair (BER) is initiated by DNA repair enzymes known as DNA glycosylases that cleave the glycosylic bond between the base and deoxyribose of damaged specific nucleotides. The phosphate backbone adjacent to the apurinic/apyrimidinic (AP) site is then cleaved by an AP endonuclease or glycosylase-associated AP lyase. These enzymes can be used in vitro to detect specific forms of DNA damage. Trevigen’s unique FLARE™ Assays characterize DNA damage in single cells using a variety of DNA repair enzymes in combination with the comet assay to recognize oxidative damage.
DNA Repair Enzymes (6)
A variety of DNA repair enzymes (Fpg, hOGG1, MutY, TDG and Endo III) are available as individual components with optimized assay conditions and reaction buffers for research in areas of base excision repair and oxidative damage. Trevigen also offers the thermophilic DNA polymerase Dpo4 capable of performing translesion synthesis for coupling to PCR for amplification of damaged DNA.
Stand-alone Reaction Buffers (6)Optimized Enzyme Reaction Buffers (10X REC™ Buffer) and cations for use with Trevigen’s DNA Repair Enzymes.
DNA Repair Enzymes Enzyme Reaction Buffers Dpo4 Polymerase 10X REC™ Buffer 15/100 mM MgCl2/50 mM MnCl2 E. coli Endonuclease III 10X REC™ Buffer 4 E. coli Fpg 10X REC™ Buffer 10 E. coli MutY 10X REC™ Buffer 4 hOGG1 10X REC™ Buffer 6 Thermostable TDG 10X REC™ Buffer 4