Standard Comet Assay (CometAssay®)

In the standard comet assay, cells are immobilized in a bed of low melting point agarose on a specially treated microscope slide. Following gentle cell lysis, and for the alkaline comet assay, samples are treated with alkali to unwind and denature the DNA and hydrolyze sites of damage. The remaining nucleoids are subjected to electrophoresis and subsequent staining with a fluorescent DNA intercalating dye. Trevigen provides a complete standardized system for consistent results which includes CometAssay® Kits, CometSlides™, Alkaline and Neutral CometAssay® Control Cells and a specialized electrophoresis unit.

Contract Services are also available for the Comet Assay.

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