Assay Kits (6)
Trevigen offers kits that measure the in vivo and in vitro activities of PARP. The Homogeneous Inhibition Assay Kit for PARP 1 is well suited for the large-scale screening of compound libraries. Our Universal Assay Kits for PARP 1 are designed to analyze small numbers of inhibitors providing accurate IC50 information. Once inhibitors of PARP 1 have been identified using in vitro screening assays, their in vivo activity in tissue or peripheral blood mononuclear cell extracts can be measured using Trevigen’s PARP in vivo Pharmacodynamic Assay II Kit. The PARP/Apoptosis Assay Kits measure decreasing levels of PARP 1 activity as cells move through the apoptotic pathway in cell line extracts. Tankyrase 1 (PARP 5A) Activity Assay Kits are also available for the screening of Tankyrase 1 inhibitors and determining IC50 values.
Trevigen offers purified human enzymes for PARP-1, Tankyrase-1, and PARG for inhibitor studies and drug discovery applications.
NAD+ substrates (2)
Poly (ADP-ribose) polymerases (e.g. PARP-1, PARP-2), and the SIR2 family of NAD+-dependent histone/protein deacetylases, use NAD+ as a substrate for their function. In addition to NAD+, Trevigen offers Biotinylated NAD+ and Fluorescein-NAD+ for detection of PARP activity.
Purified Poly ADP-ribose (PAR) Polymers (2)
Trevigen offers purified poly ADP-ribose (PAR) polymer and Biotin (terminal) PAR polymer cleaved from PARP and free of NAD+.
PAR and PARP Antibodies (7)
The PAR monoclonal and polyclonal antibodies are specific for PAR polymers synthesized by a variety of PARP isoforms (PARP 1, 2, 3, tankyrase, vPARP, and others).
The PARP monoclonal antibody can be used for analysis of PARP protein levels and cleavage of PARP in apoptotic cells. It recognizes both the full-length protein (116 kDa) and an 85 kDa cleavage fragment associated with apoptosis.
Stand-alone Assay Reagents (5)
Components available for individual purchase from the Universal Assay Kits.