TUNEL Based Kits

DNA fragmentation occurs as one of the final stages of programmed cell death and has long been considered a hallmark of apoptosis as well as one of the defining biochemical events of the pathway. DNA fragmentation can be detected in situ within the nuclei of fixed cells and tissues where the integrity of the DNA and the free 3’ hydroxyl groups at the sites of cleavage have been preserved by fixation.
The DNA provides a target for terminal deoxynucleotidyl transferase (TdT), which can sequentially add nucleotides to the 3’ end at the break. The addition of a nucleotide tagged with biotin (biotin-dNTP) or BrdU in the reaction mix then allows indirect visualization of any DNA labeling by TdT. Trevigen has developed a series of TUNEL-based assay kits for the in situ detection of apoptosis with colorimetric and fluorometric options. The biotin-dNTP based TACS® kits  and BrdU based TACS•XL® and DermaTACS™ are tailored for the detection of DNA fragmentation associated with apoptosis in a variety of cell and tissue types and for analysis by different formats that include microscopy, flow cytometry, and 96 well plates.

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